It is important to detect both above-mentioned antibodies in people with weakened immune system. to IFA method, 46 (41.8?%) and 3 (2.7?%) of diabetic ladies were positive for IgG and IgM antibodies, respectively, while in control group, 21 individuals (19.09?%) were positive for IgG antibody. In this method, IgM antibody was bad for those samples of control group (0?%). In both methods, the values acquired in the case group were significantly higher than those in the control group (IgG and IgM antibodies in diabetic pregnant women was higher than that in non-diabetic pregnant women. It seems that testing tests for looking for individuals and teaching the transmission routes should be considered as prenatal cares for diabetic ladies. is an Ruxolitinib sulfate intracellular parasite that has a worldwide distribution. Pet cats and felids are its definitive hosts and humans, many hiap-1 warm-blooded vertebrates and parrots are its intermediate hosts. has different forms of trophozoite, tissue cyst and oocyst. Oocysts are created in the intestine of the Ruxolitinib sulfate definitive sponsor and are excreted in the feces. Humans are infected through the consumption of undercooked meat containing cells cysts or the ingestion of food or water contaminated with oocyst defecated by pet cats. Toxoplasmosis in immunocompetent individuals is definitely often clinically asymptomatic. Ingesting contaminated foods, bradyzoites and sporozoites are released in the intestine, enter small intestine through epithelial and transform into tachyzoites which undergo replication and spread in the body. This parasite can lead to chronic illness in adults, death in immunocompromised people and miscarriage in pregnant women. The prevalence of varies in different societies depending on the geographical region (Robert-Gangneux and Darde 2012). For example, the prevalence rate of in pregnant women in Portugal is definitely 59.8?% and in pregnant women in Turkey (52.1?%) (Ocak et al. 2007; Varella et al. 2003). In addition, this coccidian protozoan is definitely widely distributed in Iran and the prevalence rate in Khuzestan ranged from 21.5 to 47.25?% (Daryani et al. 2014; Khademvatan et al. 2013; Saki et al. 2013, 2015; Soltani et al. 2013; Yad et al. 2014). It seems that the oocytes excreted by pet cats find the opportunity to become infectious due to its warm-humid months in khuzestan (Khademvatan et al. 2014). Appropriate analysis of toxoplasmosis is vital among the individuals: Pregnant women who have acquired this illness during pregnancy, babies who have congenitally acquired toxoplasmosis during the prenatal period, individuals with weakened immune system, and Ruxolitinib sulfate chorioretinitis individuals (Ghasemian et al. 2007; Saki et al. 2015). The probability of prenatal transmission raises as the fetal age increases; so that it is definitely 10C15, 30C54 and 60C65?% during the first, second and third trimesters of pregnancy, respectively. The infected fetus may show a wide range of medical symptoms of varying severity, from miscarriage and severe illness of neonates to asymptomatic infections (Neva and Brownish 1994). This parasite functions as an opportunistic pathogen in immunocompromised people such as those with HIV, encephalitis and systemic illness (Zangerle et al. 1991). Several studies have shown that in diabetic patients, leukocyte cytotoxicity to remove pathogenic factors is definitely reduced and the opsonic activity of immune system is definitely significantly diminished; consequently, diabetic pregnant women are one of the organizations susceptive to such opportunistic infections (Rayfield et al. 1982). Confirmation of analysis of infection is necessary by laboratory checks. Although, the number of instances with toxoplasmosis is definitely high, a sensitive and appropriate method is not applied for detection of this illness in most diagnostic laboratories, which will lead to mismanagement of the involved instances. Among serologic methods, Enzyme-linked Immunoadsorbent Assay (ELISA), immunofluorescence assay (IFA), and indirect hemagglutination Test (IHT) are standard methods for analyzing anti-antibodies (Robert-Gangneux and Darde 2012); consequently, using ELISA and IFA methods with this study, we intend to compare two mentioned methods as well as investigate IgG and IgM antibodies produced against in diabetic pregnant women of Ahvaz city, southwest of Iran country. Materials and methods With this caseCcontrol study blood samples were collected from ladies, who have been registered as diabetic patients and underwent regular monthly follow ups by genecology professional in the state and private hospitals in Ahvaz city southwest of Iran. A total.